Question 1

Genetic engineering methods have been used to produce vaccines.

Accepted Answer

A) True 
 B)False

Question 2

When a eukaryotic gene is expressed in a bacterium,the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.

Accepted Answer

A) True 
 B)False

Question 3

Complementary DNA (cDNA) probes are produced using

Accepted Answer

A)  restriction endonucleases.
B)  RNA polymerase.
C)  DNA ligase.
D)  reverse transcriptase.E) A) and B)
F) A) and C)

Question 4

A DNA molecule used to carry a foreign gene into a host organism is called a

Accepted Answer

A)  plasmid.
B)  vector.
C)  probe.
D)  blot.E) A) and B)
F) All of the above B

Question 5

In order to express eukaryotic genes in a bacterium,the __________ must first be removed.

Accepted Answer

A)  introns
B)  exons
C)  enhancers
D)  3' poly A sequenceE) A) and B)
F) All of the above

Question 6

Transposons are frequently used as cloning vectors.

Accepted Answer

A) True 
 B)False

Question 7

In _____________,cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Accepted Answer

The answer of In _____________,cells are mixed with recombinant DNA...

Question 8

Regardless of the exact approach taken to recombinant DNA technology,one of the keys to successful cloning is choosing the right vector.

Accepted Answer

A) True 
 B)False  True

Question 9

A PCR procedure that allows a determination of the amount of a particular DNA fragment that is present in a sample is called

Accepted Answer

A)  quantitative PCR.
B)  analytical PCR.
C)  real-time PCR.
D)  reverse PCR.E) All of the above
F) A) and C)

Question 10

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

Accepted Answer

A)  ligation.
B)  transformation.
C)  transduction.
D)  plasmolysis.E) B) and D)
F) None of the above

Question 11

Plasmid vectors often contain __________ genes that can be used to screen for recombinants.

Accepted Answer

A)  metabolic activation
B)  antibiotic resistance
C)  insertion sequence
D)  promoter/operatorE) A) and C)
F) A) and B)

Question 12

Recombinant DNA technology does not rely on which of the following enzymes?

Accepted Answer

A)  restriction endonucleases
B)  RNA methylase
C)  DNA ligase
D)  reverse transcriptaseE) A) and B)
F) None of the above

Question 13

When a eukaryotic gene is cloned into a bacterium,the advantage of a complementary DNA (cDNA) gene being used instead of fragments of genomic DNA is that

Accepted Answer

A)  the promoter and terminator are found in the cDNA gene but not in the genomic fragment.
B)  the introns have been removed from the cDNA gene but not from the genomic fragment.
C)  the cDNA is made with the nucleotides found in the prokaryote but not in the eukaryote.
D)  there is no advantage to using a cDNA gene rather than a genomic fragment.E) B) and C)
F) None of the above B

Question 14

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

Accepted Answer

A) True 
 B)False

Question 15

Restriction endonucleases in bacteria may have evolved in order to

Accepted Answer

A)  carry out natural genetic engineering.
B)  protect the bacteria from infection by viruses.
C)  use nucleic acids as a food (energy) source.
D)  all of the choicesE) A) and B)
F) None of the above

Question 16

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

Accepted Answer

A)  bacterial artificial chromosome.
B)  bacteriophage.
C)  cosmid.
D)  plasmid.E) A) and B)
F) A) and C)

Question 17

A (n) __________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

Accepted Answer

A)  shuttle
B)  chimeric
C)  expression
D)  phageE) A) and D)
F) C) and D)

Question 18

A(n)__________ __________ is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb)pieces of foreign DNA into a host organism.

Accepted Answer

artificial...

Question 19

The enzyme reverse transcriptase was discovered by

Accepted Answer

A)  Arber and Smith.
B)  Jackson,Symons,and Berg.
C)  Boyer and Cohen.
D)  Temin and Baltimore.E) A) and B)
F) A) and C)

Question 20

The three steps that take place in each cycle during PCR occur in which order?

Accepted Answer

A)  DNA annealing,denaturation,and synthesis.
B)  DNA denaturation,annealing,and synthesis.
C)  DNA synthesis,denaturation,and annealing.
D)  none of the above.E) A) and D)
F) A) and C)

Exam 17: Recombinant Dna Technology

Genetic engineering methods have been used to produce vaccines.

Correct AnswerverifiedShow Answer

When a eukaryotic gene is expressed in a bacterium,the eukaryotic regulatory sequences should be maintained in order to achieve maximum expression of the gene.

Correct AnswerverifiedShow Answer

Complementary DNA (cDNA) probes are produced using

Correct AnswerverifiedShow Answer

A DNA molecule used to carry a foreign gene into a host organism is called a

Correct AnswerverifiedB

In order to express eukaryotic genes in a bacterium,the __________ must first be removed.

Correct AnswerverifiedShow Answer

Transposons are frequently used as cloning vectors.

Correct AnswerverifiedShow Answer

In _____________,cells are mixed with recombinant DNA and exposed to a brief pulse of high-voltage electricity to cause the membrane to become permeable and allow the uptake of DNA from its environment.

Correct AnswerverifiedShow Answer

Regardless of the exact approach taken to recombinant DNA technology,one of the keys to successful cloning is choosing the right vector.

Correct AnswerverifiedTrue

A PCR procedure that allows a determination of the amount of a particular DNA fragment that is present in a sample is called

Correct AnswerverifiedShow Answer

Plasmid cloning vector DNA is usually introduced into bacterial hosts by

Correct AnswerverifiedShow Answer

Plasmid vectors often contain __________ genes that can be used to screen for recombinants.

Correct AnswerverifiedShow Answer

Recombinant DNA technology does not rely on which of the following enzymes?

Correct AnswerverifiedShow Answer

When a eukaryotic gene is cloned into a bacterium,the advantage of a complementary DNA (cDNA) gene being used instead of fragments of genomic DNA is that

Correct AnswerverifiedB

One of the major advantages to using plasmids as cloning vectors is that very high copy numbers can be achieved with many types of plasmid vectors.

Correct AnswerverifiedShow Answer

Restriction endonucleases in bacteria may have evolved in order to

Correct AnswerverifiedShow Answer

Which of the following types of cloning vector can carry the largest amount of foreign DNA?

Correct AnswerverifiedShow Answer

A (n) __________ vector contains promoters that result in high-level transcription of the gene cloned within a multicloning site.

Correct AnswerverifiedShow Answer

A(n)__________ __________ is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb)pieces of foreign DNA into a host organism.

Correct Answerverifiedartificial...View AnswerShow Answer

The enzyme reverse transcriptase was discovered by

Correct AnswerverifiedShow Answer

The three steps that take place in each cycle during PCR occur in which order?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
B

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
True

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
B

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

A(n) is a piece of DNA with all of the features necessary for chromosomal replication and which can carry large (up to 1000 kb)pieces of foreign DNA into a host organism.

Correct Answer
verified
artificial...
View Answer

Correct Answer
verified

Correct Answer
verified