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Which of the following is not part of a yeast artificial chromosome (YAC) ?


A) The F factor.
B) A selectable marker.
C) An ARS.
D) A CEN sequence.

E) B) and C)
F) A) and B)

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A (n) __________ vector is a plasmid that can be replicated in several different organisms because it has at least one origin of replication that will function in each host.


A) shuttle
B) chimeric
C) expression
D) phage

E) A) and D)
F) A) and C)

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The polymerase chain reaction (PCR) can be used to produce __________ of copies in a few hours.


A) hundreds
B) thousands
C) millions
D) billions

E) A) and D)
F) A) and C)

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Which of the following was first produced commercially using recombinant DNA technology?


A) Human growth hormone.
B) Interleukins.
C) Hepatitis B vaccine.
D) Human insulin.

E) B) and C)
F) A) and D)

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Promoters for genes that code for proteins can be isolated from a cDNA library.

A) True
B) False

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Which of the following can be used as vectors for cloning DNA fragments?


A) plasmids.
B) cosmids.
C) bacteriophages.
D) all of the choices.

E) A) and B)
F) None of the above

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Restriction endonucleases were discovered by


A) Arber and Smith.
B) Jackson,Symons,and Berg.
C) Boyer and Cohen.
D) Temin and Baltimore.

E) A) and D)
F) A) and C)

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