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Peptidoglycan is a unique macromolecule found in bacterial ______.


A) slime layers
B) capsules
C) cell walls
D) cell membranes
E) inclusions

F) All of the above
G) A) and C)

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The function of bacterial endospores is


A) to provide a protected site for photosynthesis.
B) storage of excess cellular building blocks.
C) protection of genetic material during harsh conditions.
D) reproduction and growth.

E) A) and C)
F) B) and C)

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Endospores are


A) metabolically inactive.
B) resistant to heat and chemical treatments.
C) resistant to destruction by radiation.
D) living structures.
E) All of the choices are correct.

F) A) and E)
G) B) and E)

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Which of the following bacterial structures is incorrectly matched with a function?


A) Nucleoid - hereditary material
B) Plasmids - contain genes essential for growth and metabolism
C) Cytoplasm - dense, gelatinous solution
D) Ribosomes - protein synthesis
E) Inclusions - excess cell nutrients and materials

F) All of the above
G) A) and B)

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You need to determine if a pure culture of bacteria is gram-positive or gram-negative,but you've just spilled your only solution of crystal violet so you can't do a Gram stain.You decide to try a lysozyme treatment on a sample of each culture and then examine the samples under the microscope.The expected result is


A) gram-positives will lyse and appears as debris; gram-negatives will lyse but more slowly.
B) gram-positives will lyse and appears as debris; gram-negatives will be unaffected.
C) gram-positives will lyse and appears as debris; gram-negatives will lose cellular morphology and appear as cocci.
D) gram-negatives will lyse and appears as debris; gram-positives will lose cellular morphology and appear as cocci.
E) gram-negatives will lyse and appears as debris; gram-positives will be unaffected.

F) All of the above
G) A) and B)

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Bacterial cells could have any of the following appendages except ______.


A) cilia
B) fimbriae
C) periplasmic flagella (axial filaments)
D) flagella
E) pili

F) None of the above
G) A) and D)

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Chemical analysis of a bacterial cell detects dipicolinic acid.What is the identity of this structure?


A) Nucleoid
B) Cell wall
C) Biofilm
D) Capsule
E) Endospore

F) A) and E)
G) A) and B)

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A nutrient binds to receptors near the flagellar basal body.This will result in


A) inhibition of flagella rotation.
B) numerous tumbles.
C) clockwise rotation of flagella.
D) counterclockwise rotation of flagella.

E) C) and D)
F) All of the above

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The term diplococcus refers to an irregular cluster of spherical bacterial cells.

A) True
B) False

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A bacterial cell wall that has primarily peptidoglycan with small amounts of teichoic acid and lipoteichoic acid is ______.


A) a spheroplast
B) acid-fast
C) gram-negative
D) gram-positive
E) a protoplast

F) A) and E)
G) B) and E)

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Some bacteria have a cytoskeleton of sterols to help maintain their shape.

A) True
B) False

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Chemotaxis refers to the ability of a cell to


A) move in response to light.
B) move in response to a chemical.
C) transport desired molecules into a cell.
D) halt movement in response to a chemical.

E) A) and C)
F) B) and C)

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Researchers studying the rigidity and flexibility of the cell envelope used chemotaxis to measure the ability of microbes to squeeze through tight spaces.E.coli,a gram-negative bacterium,was able to traverse narrower channels than B.subtilis,a gram-positive microbe,even though both cells are the same size when grown without restriction.E.coli has the more flexible cell envelope because


A) lipopolysaccharide has no charge.
B) it has fewer flagella.
C) it has only a single membrane.
D) there is less structural carbohydrate.
E) there are fewer protein receptors.

F) A) and B)
G) C) and D)

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A bacterial cell exhibiting chemotaxis must have ______.


A) a capsule
B) flagella
C) thylakoids
D) metachromatic granules
E) fimbriae

F) B) and D)
G) B) and C)

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The cell envelope of gram-positive bacteria has two layers: a thick cell wall and the cell membrane.

A) True
B) False

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You have made a smear of a bacterial culture and have performed the Gram stain on it.Looking at the organism under the microscope,you notice that the cells do not seem to be the dark blue-purple of a gram-positive reaction,but instead are light purple.Your staining procedure was performed correctly.What is your best explanation as to why the bacteria have stained this way?


A) The bacteria were taken from an inappropriate medium, and a chemical is interfering with proper staining of the cells.
B) There is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall, thereby staining it improperly.
C) The specimen did not undergo heat fixation before staining; therefore, the primary dye does not stick properly to the wall.
D) These are mutant bacteria which cannot be stained like other bacteria.

E) C) and D)
F) None of the above

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The cell membrane,the cell wall,and the outer membrane comprise the ______.


A) cell envelope
B) slime layer
C) glycocalyx
D) S-layer
E) Peptidoglycan

F) C) and D)
G) B) and C)

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Serological analysis for bacterial identification typically involves using


A) specific antibodies to the bacterial cell antigens.
B) a microscope to determine cell morphology.
C) the determination of guanine + cytosine base concentrations.
D) methods to identify cell enzymes.
E) the analysis of the appearance of colonies.

F) C) and D)
G) All of the above

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The bacterial cell membrane is a site for many enzymes and metabolic reactions.

A) True
B) False

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Plasmids


A) are found in all bacteria.
B) are essential for survival.
C) are located in microcompartments.
D) cannot be passed between organisms.
E) often carry genes controlling pathogenicity.

F) A) and E)
G) A) and C)

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